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Journal: Research
Article Title: Mapping Immune-Inflammatory Niches on Zirconia Bone Implants: Single-Cell Transcriptomic Profiling
doi: 10.34133/research.1162
Figure Lengend Snippet: Ti and ZrO 2 implants induce early osseointegration and immune inflammation. (A) Transcriptional levels of osteogenesis-related and immune-inflammatory genes. Osteogenic genes: Runx2 , Osterix , and Tgfb1 . Immune-inflammatory genes: Il1b , Mmp9 , and Il23a . (B) Receiver operating characteristic (ROC) analysis based on bulk RNA-seq data. AUC, area under the curve. (C) Protein expression levels of osteogenesis-related and immune-inflammatory markers. Osteogenic proteins: RUNX2, OSX, TGFB1, pSMAD2/SMAD2, and pSMAD3/SMAD3. Immune-inflammatory proteins: IL1B, MMP9, and IL23A. (D) Statistical analysis of protein expression levels. Statistical significance was determined using one-way analysis of variance. RUNX2, RUNX family transcription factor 2; OSX, osterix; TGFB1, transforming growth factor beta 1; SMAD2, SMAD family member 2; SMAD3, SMAD family member 3; IL1B, interleukin 1 beta; MMP9, matrix metallopeptidase 9; IL23A, interleukin 23 subunit alpha.
Article Snippet: The membranes were blocked with 5% nonfat milk for 1 h, followed by overnight incubation at 4 °C with the following primary antibodies: RUNX2 (Bioss, bs-1134R, 1:1,000, China), OSX (Bioss, bs-25532R, 1:1,000, China), pSMAD2 (Bioss, bs-3419R, 1:1,000, China),
Techniques: RNA Sequencing, Expressing